The EFS liquid shot-shelf life is dependent on use. The jug, when poured into a flask or any other container is stable for 18 months from the date of manufacture. The expiration date is listed on the bottom. This shelf life is based on room temperature, though First Endurance does recommend refrigeration as an additional precaution. The flask shelf-life starts the minute you put your lips to the bottle. Because this introduces bacteria and because this is typically done during hot training sessions, First Endurance recommends consumption within 24 hours. Following your workout you can clean the flask and re-fill from the Jug.